Estimation of oxyhaemoglobin and of methaemoglobin by a photoelectric method.

THE oxyhaemoglobin content of blood can be rapidly and accurately estimated with the help of a photoelectric colorimeter. In the absence of oxyhaemoglobin or of other pigments, methaemoglobin can be determined in the same way. In whole blood the presence of metor of sulph-haemoglobin or of other pigments can be detected. The experiments also indicate that the use of 084% ammonia as a diluent for blood for spectrophotometric measurements is not safe. Better results can be obtained with a concentration of 0 04% ammonia.